Abstract
Objectives: MBL (Mannose-binding lectin) is a protein made predominantly inside of the liver, which linked mannose, N-acetyl glucosamine, and focus residues on the cell walls of the microorganism. We saw that the polymorphisms of (MBL2 gene) affect host sensitivity to Candida infection. Human leucocyte antigen-G (HLA-G) are an insertion/deletion polymorphism play a role as immunotolerance, stimulate apoptosis of activated CD8+ T cells, interactions with the (T regulatory cells), modification of the action of dendritic cells and natural killer cells. The polymorphisms in these genes have been reported in several diseases. To evaluate the impact of MBL-2, HLA-G (Ins/Del) polymorphisms in Vulvovaginal candidiasis (VVC) Iraqi patients women.

Material and Method: Fifty four blood samples from each (VVC)patients women and healthy control group was collected, then DNA was extracted and analyzed for MBL-2 and HLA-G genotypes and Alleles frequencies with (PCR) and by Gel electrophoreses using 2.25%, 3% Agarose concentration (respectively) was examined.

Results: The result revealed that 6(12%) patient women infected with vulvovaginal candidiasis has bb allele, and 36(72%) patients infected with vulvovaginal candidiasis has aa allele which referred as wild type, while 8(16%) patients has Ab allele which consider as mutant. There was significant increasing in the bb genotype and Ab genotype frequency in candida patients and increase infection with vulvovaginal candidiasis and b allele consider as a risk factor. *P 0.008*, 0.02*; OR= 0.42(0.33-0.54), 5.33(1.06-26.64). For HLA-G gene polymorphism has 13(26%) patients has Deletion / Deletion genotype, 20(40%) patients has Insertion/ Deletion genotype while 17(34%) patients has Insertion / Insertion genotype, there was significant relation between HLA-G genotype and vulvovaginal candidiasis infection p=0.01,, we found significant increase of D/D genotype in controlled group when compared with patients p=0.03, OR=0.34 CI;(0.12-0.98) which suggests an increase protective effect of D/D genotype against the vulvovaginal candidiasis infection.

Conclusion: There was significant relation between MBL-2 and vulvovaginal candidiasis patients women it consider as a risk factor, so there was also significant relation between HLA-G and vulvovaginal candidiasis patients women it consider as a protective effect.

Key words: Vulvovaginal Candidiasis (VVC), MBL-2, HLA-G Polymorphism.