Abstract
To appraise the association of VDR FokI and TaqI polymorphisms with diabetic nephropathy patients, a total of 60 men with type 2 diabetes mellitus (T2DM) were enrolled in the recent paper, as well as 17 healthy men as a control. The patients have been divided according to the albumin/creatinine ratio (Normo albuminuria, micro-albuminuria, and macro-albuminuria,). ARMS PCR was used to diagnose the genotypes and allele frequencies of FokI "rs10735810*" and TaqI "rs731236*". The results indicated that FF, Ff and TT, Tt genotype were significantly higher in patients with T2DM than the control (OD=3.375, p=0.001), (OD=4.333, p=0.001), (OD=4.222, p=0.001), and (OD=2.833, p=0.025) respectively. Moreover, the results showed a notable increase of FF, Ff, and TT, Tt genotypes in the patients with micro-albuminuria and macro-albuminuria when compared with patients of normo albuminuria, (p=0.002), (p=0.005), (p=0.001), (p=0.010) respectively. The current outcomes propose that the F allele of FokI and T allele of TaqI polymorphisms in the VDR gene are associated with a higher risk of T2DM and nephropathy stages. Futhermore, the FokI and TaqI can be used as vital markers to predict the risk of complications of diabetes mellitus especially nephropathy.

Key words: ARMS-PCR, Diabetes Type 2, Diabetes Nephropathy, VDR Gene Polymorphisms.