E-ISSN 0976-2833 | ISSN 0975-3583
 

Original Research 


Age-Related Changes in Lymph Nodes

& 1040;leksei V. Tverskoi, Vitaliy N. Morozov, & 1045;lena N. Morozova, & 1040;nastasya V. Tverskaya, Evgeniy A. Yatsenko, Kseniya V. Rossiyskaya.

Abstract
Lymph nodes are important secondary lymphoid organs in which immunocompetent cells can quickly respond to blood and lymph pathogens, creating long-term adaptive immune responses. With age, structural changes that occur affect the functioning of immunocompetent cells, which may ultimately lead to less effective or reduced immune responses.The purpose was to study the morphological features of lymph nodes in people aged 65-69 years and to analyze the results with data from other researchers. The study was carried out on 30 lymph nodes obtained during routine autopsy of people aged 65-69 years. Lymph nodes were subjected to standard histological protocol; slides were stained with hematoxylin and eosin. An immunohistochemical study was performed to determine the expression of the markers Ki-67, p53, bcl-2 and Cyclin B1. The thickening of the capsule and connective tissue trabecules were determined on the sections of the lymph nodes, the cleavage of trabecules by adipocytes. The zonal structure of the lymph node is smoothed. Lymphatic nodules in the cortex have unclear contours, they do not have germinal centers. The boundaries of the internodular zone and paracortex are not determined. The marginal sinus is unevenly expanded. In the medulla, medullary cords are replaced by white adipose tissue. The medullary sinuses are dilated, adipocytes are located in the lumen of some of them. Small arteries and arterioles with thickened walls and signs of hyalinosis occur. An immunohistochemical study of the proliferative activity of lymph node cells shows a significant decrease in the number of positively stained lymphocytes in both the cortex and medulla by the expression of the Ki-67 marker. In a study of the expression of Cyclin B1 and p53 receptors, the reaction is negative in both cases, while a reaction with antibodies to bcl-2 revealed weak cytoplasmic staining of lymphocytes of the cortex and medulla. Own and literature data shows that with age, unidirectional degenerative and dystrophic changes occur in the lymph nodes of humans, rats and mice.This resultsin increase in the proportion of connective and adipose tissue and a decrease in lymphoid tissue in the lymph nodes. These changes can be considered morphological signs of inhibition of lymphocytopoiesis, humoral and cellular immunity in the lymph nodes.

Key words: lymph node; cortex; medulla; Ki-67, p53, CyclinB1, bcl-2markers; age changes


 
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How to Cite this Article
Pubmed Style

a, , lNMa, . Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12


Web Style

a, , lNMa, . Age-Related Changes in Lymph Nodes . http://www.jcdronline.org//?mno=6660 [Access: October 07, 2020]. doi:10.31838/jcdr.2020.11.04.12


AMA (American Medical Association) Style

a, , lNMa, . Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12



Vancouver/ICMJE Style

a, , lNMa, . Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12



Harvard Style

, . a. ., , , l. N. M. a. . & (2020) Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12



Turabian Style

, and , leksei V. Tverskoi, Vitaliy N. Morozov, and, lena N. Morozova, and, and nastasya V. Tverskaya, Evgeniy A. Yatsenko, Kseniya V. Rossiyskaya. 2020. Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12



Chicago Style

, and , leksei V. Tverskoi, Vitaliy N. Morozov, and, lena N. Morozova, and, and nastasya V. Tverskaya, Evgeniy A. Yatsenko, Kseniya V. Rossiyskaya. "Age-Related Changes in Lymph Nodes ." doi:10.31838/jcdr.2020.11.04.12



MLA (The Modern Language Association) Style

, and , leksei V. Tverskoi, Vitaliy N. Morozov, and, lena N. Morozova, and, and nastasya V. Tverskaya, Evgeniy A. Yatsenko, Kseniya V. Rossiyskaya. "Age-Related Changes in Lymph Nodes ." doi:10.31838/jcdr.2020.11.04.12



APA (American Psychological Association) Style

, . a. ., , , l. N. M. a. . & (2020) Age-Related Changes in Lymph Nodes . doi:10.31838/jcdr.2020.11.04.12





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