A VALIDATED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SIMULTANEOUS METHOD FOR THE QUANTIFICATION OF IMATINIB MESYLATE AND ANASTRAZOLE BY PDA DETECTOR

Abstract

The aim of the project is to develop a fast, simple, precise, and cost-efficient RP-HPLC 
method for measuring the amounts of Imatinib mesylateand Anastrazole simultaneously in 
both pharmaceutical products and bulk samples. This technique has effectively achieved the 
separation of Imatinib mesylateand Anastrazole in large quantities. The separation was 
performed using a Phenomenex C18 150 x 4.6 mm, 5m analytical column at a wavelength of 
280 nm. The mobile phase consisted of a mixture of methanol, water and acetonitrile in a 
ratio of 30:60:10. The pH of the buffer was adjusted to 5.5. The separation was carried out in 
isocratic elution mode with a flow rate of 1.0 ml/min. Imatinib mesylate had a retention time 
of 1.885 minutes, whereas Anastrazole showed a retention duration of 3.139 minutes.