E-ISSN 0976-2833 | ISSN 0975-3583
 

Research Article 


PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL

Amandeep Singh, Nisha A Bhatt, Mohd. Adnan.

Abstract
The objective of the present project work was to investigate the active constituent of plant Piper betel
characterized for its antimicrobial activity. For the research analysis the stem powder subjected to phytochemical
screening and was found to contain carbohydrate, protein, polyphenolic compounds, flavonoid, alkaloids and total
antioxidant. The estimation and evaluation of antimicrobial activity was done by disc diffusion method. For this the
different concentration ethanolic extract (20, 40, 60 & 80%) was prepared and from which initial concentration of
1mg/ml were loaded on 6mm dimension filter paper which was sterilized previously by autoclaving. The loaded disc
was placed on surface of medium and the compound was allowed to diffuse for 5 minutes and all the plated were
incubated at 37℃ for 24 hrs. The end of incubation, inhibition zones formed around the disc in few mm,
measurement done by using the ruler. As, a result the maximum inhibition was showed by the concentration of 60%
dilution. The extract confirmed significant antimicrobial activity against the bacterial strains test.

Key words: The objective of the present project work was to investigate the active constituent of plant Piper betel characterized for its antimicrobial activity


 
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Pubmed Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. J Cardiovasc. Dis. Res.. 2021; 12(5): 224-229. doi:10.31838/jcdr.2021.12.05.34


Web Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. http://www.jcdronline.org/?mno=107987 [Access: August 09, 2021]. doi:10.31838/jcdr.2021.12.05.34


AMA (American Medical Association) Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. J Cardiovasc. Dis. Res.. 2021; 12(5): 224-229. doi:10.31838/jcdr.2021.12.05.34



Vancouver/ICMJE Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. J Cardiovasc. Dis. Res.. (2021), [cited August 09, 2021]; 12(5): 224-229. doi:10.31838/jcdr.2021.12.05.34



Harvard Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan (2021) PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. J Cardiovasc. Dis. Res., 12 (5), 224-229. doi:10.31838/jcdr.2021.12.05.34



Turabian Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. 2021. PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. Journal of Cardiovascular Disease Research, 12 (5), 224-229. doi:10.31838/jcdr.2021.12.05.34



Chicago Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. "PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL." Journal of Cardiovascular Disease Research 12 (2021), 224-229. doi:10.31838/jcdr.2021.12.05.34



MLA (The Modern Language Association) Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan. "PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL." Journal of Cardiovascular Disease Research 12.5 (2021), 224-229. Print. doi:10.31838/jcdr.2021.12.05.34



APA (American Psychological Association) Style

Amandeep Singh, Nisha A Bhatt , Mohd. Adnan (2021) PHYTOCHEMICAL SCREENING & IN-VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY FROM STEM EXTRACT OF PLANT PIPER BETEL. Journal of Cardiovascular Disease Research, 12 (5), 224-229. doi:10.31838/jcdr.2021.12.05.34





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