E-ISSN 0976-2833 | ISSN 0975-3583
 

Research Article 


Detection of yeast species associated with respiratory tract infections using internal transcript spacer

Iman .H.al fayyadh, Mohammed Hashim al-yasiri.

Abstract
Fungal infections have increased during last two years to become a grown threat due to the prevalence of
COVID-19, which is the first factor to inhibit the immune system. Therefore, this study aimed to screen
fungal species associated with respiratory infections using internal transcript spacer as diagnostic marker.
The total samples collected in this study were 160 samples of sputum, which included filamentous fungi
and yeasts. The total number of positive samples was 124 (77.5%) which included 62 yeast positive
samples, and 62 filamentous positive samples. Yeast samples included different genera and the highest
percentage belonged to the genus Candida. Five species of the genus Candida, C. tropical. C. albicans, C.
dubliniensis, C. krusei and C. glabrata were identified based on phenotypic characteristics and biochemical
tests as well as using chromogenic Candida agar medium. The highest percentage of isolated yeasts was
Candida yeast 53, with a rate of )85.48%( out of 62 samples of yeasts.The highest percentage of yeasts was
Candida albicanis, which amounted to 26 (41.93) positive samples for examination according to the
genetic diagnosis of yeasts

Key words: Candida, respiratory system, biochemical diagnosis, DNA sequencing


 
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Pubmed Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. Detection of yeast species associated with respiratory tract infections using internal transcript spacer. J Cardiovasc. Dis. Res.. 2021; 12(5): 962-966. doi:10.31838/jcdr.2021.12.05.127


Web Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. Detection of yeast species associated with respiratory tract infections using internal transcript spacer. http://www.jcdronline.org/?mno=122511 [Access: September 10, 2021]. doi:10.31838/jcdr.2021.12.05.127


AMA (American Medical Association) Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. Detection of yeast species associated with respiratory tract infections using internal transcript spacer. J Cardiovasc. Dis. Res.. 2021; 12(5): 962-966. doi:10.31838/jcdr.2021.12.05.127



Vancouver/ICMJE Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. Detection of yeast species associated with respiratory tract infections using internal transcript spacer. J Cardiovasc. Dis. Res.. (2021), [cited September 10, 2021]; 12(5): 962-966. doi:10.31838/jcdr.2021.12.05.127



Harvard Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri (2021) Detection of yeast species associated with respiratory tract infections using internal transcript spacer. J Cardiovasc. Dis. Res., 12 (5), 962-966. doi:10.31838/jcdr.2021.12.05.127



Turabian Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. 2021. Detection of yeast species associated with respiratory tract infections using internal transcript spacer. Journal of Cardiovascular Disease Research, 12 (5), 962-966. doi:10.31838/jcdr.2021.12.05.127



Chicago Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. "Detection of yeast species associated with respiratory tract infections using internal transcript spacer." Journal of Cardiovascular Disease Research 12 (2021), 962-966. doi:10.31838/jcdr.2021.12.05.127



MLA (The Modern Language Association) Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri. "Detection of yeast species associated with respiratory tract infections using internal transcript spacer." Journal of Cardiovascular Disease Research 12.5 (2021), 962-966. Print. doi:10.31838/jcdr.2021.12.05.127



APA (American Psychological Association) Style

Iman .H.al fayyadh, Mohammed Hashim al-yasiri (2021) Detection of yeast species associated with respiratory tract infections using internal transcript spacer. Journal of Cardiovascular Disease Research, 12 (5), 962-966. doi:10.31838/jcdr.2021.12.05.127





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