E-ISSN 0976-2833 | ISSN 0975-3583
 

Research Article 


Fructosylation of Human Serum Albumin - Implications in diabetes mellitus

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin.

Abstract
Aim:To investigate the alterations in human serum albumin (HAS) due to glycation with varying fructose
concentration.
Methods:Human serum albumin (HSA)was glycated using fructose and characterized it using physicochemical
studies. The changes and modifications as a result of glycation were studied in a concentration- and time-dependent
manner.
Results:Characterization of fructosylated HSA resulted in hyperchromicity of UV spectrum, increase in AGEformation,
quenching of tryptophan fluorescenceandSDS-PAGE. Amadori products formed were estimated by
nitroblue tetrazolium. Higher carbonyl content and decreased thiol content was found in fructose modified HSA.
Diabetic sera showed increased levels of malondialdehydeand protein carbonyls indicating enhanced oxidative
stress. The diabetic seraalso showed higher recognition and binding capability with respect to glycated HSA than to
native HSA, reflecting greater specificity to glycated HSA.
Conclusion:Modification of proteins due to glycation leads to formation of epitopes that are regarded as non-self
and lead to antibody production. Structural modifications may also reflect as changes in receptor binding properties
and alteration in functions.

Key words: Fructose, Human serum albumin, diabetes, advanced glycation end products, ELISA, SDS PAGE, Epitopes


 
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How to Cite this Article
Pubmed Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. J Cardiovasc. Dis. Res.. 2021; 12(6): 356-365. doi:10.31838/jcdr.2021.12.06.46


Web Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. http://www.jcdronline.org/?mno=139103 [Access: December 04, 2021]. doi:10.31838/jcdr.2021.12.06.46


AMA (American Medical Association) Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. J Cardiovasc. Dis. Res.. 2021; 12(6): 356-365. doi:10.31838/jcdr.2021.12.06.46



Vancouver/ICMJE Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. J Cardiovasc. Dis. Res.. (2021), [cited December 04, 2021]; 12(6): 356-365. doi:10.31838/jcdr.2021.12.06.46



Harvard Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin (2021) Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. J Cardiovasc. Dis. Res., 12 (6), 356-365. doi:10.31838/jcdr.2021.12.06.46



Turabian Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. 2021. Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. Journal of Cardiovascular Disease Research, 12 (6), 356-365. doi:10.31838/jcdr.2021.12.06.46



Chicago Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. "Fructosylation of Human Serum Albumin - Implications in diabetes mellitus." Journal of Cardiovascular Disease Research 12 (2021), 356-365. doi:10.31838/jcdr.2021.12.06.46



MLA (The Modern Language Association) Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin. "Fructosylation of Human Serum Albumin - Implications in diabetes mellitus." Journal of Cardiovascular Disease Research 12.6 (2021), 356-365. Print. doi:10.31838/jcdr.2021.12.06.46



APA (American Psychological Association) Style

Dr. Sufia Naseem, Dr. Manzoor Ahmad, Ummul Baneen, Dr. Shagufta Moin (2021) Fructosylation of Human Serum Albumin - Implications in diabetes mellitus. Journal of Cardiovascular Disease Research, 12 (6), 356-365. doi:10.31838/jcdr.2021.12.06.46





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