THE ANTI-ATHEROSCLEROSIS ACTIVITY OF FUCOIDAN CRUDE EXTRACT FROM SARGASSUM CRASSIFOLIUM

Abstract

This purpose of this research is to examine the anti-atherosclerosis activity of Sargassum crassifolium brown
algae in mouse, which was administered high-fat diet, and further examining the molecular mechanism of
action, through the anti-inflammation test at RAW cell 264.7, inducted by lipopolysaccharide. Sargassum
crassifolium was collected from Garut water, extracted by acid solvent, condensed with ethanol, and freezedried till the fucoidan crude extract was obtained. However, the mouse was dosed at 50, 100, and 200 mg/kg,
after a high-fat diet, therefore, the blood lipid profile and abdominals aortae artery insulation were measured to
calculate the amount of foam cell and the aortae thickness respectively. Furthermore, fucoidan crude extract was
tested at RAW cell 264.7, which was inducted by LPS, at doses of 25 and 50 μg/ml, in order to observe its
activity, toward the TNF-α, IL-1β, IL-6, VCAM-1, and ICAM-1, measured using ELISA. Hence, the isolate
was proven to have atherosclerosis protective effect, through the improvement of blood lipid profile and the
reduction of the foam cells, as well as the thickness of abdominal aortae. Furthermore, fucoidan crude extract
obstructed the redemption of TNF-α, IL-1β, IL-6, VCAM-1, and ICAM-1 in vitro, therefore demonstrating that
its activity was through the inflammation redemption response from Sargassum crassifolium.