Molecular Typing the UropathogenicEscherichia coliClinical Strains using ERIC1b-PCR, and PCR Detection of three Candidate Biomarkers ChuA, YjaA, and TspE4C2

Abstract

The present work does address the molecular typing of ten uropathogen clinical strains isolated from
urinary tract infections (UTIs) using: ERIC1b-PCR and PCR detection of ChuA, YjaA, and TspE4C2
virulence genes. Ten out of fifty E.coliuropathogen clinical strains were isolated from patients
diagnosed as UTIs post culturing of urine samples onto blood agar and Typtone Bile X-Glucuronide
(TBX) agar plates. Results of API20E system verified the identity of these ten uropathogens as E.coli.
ERIC1b-PCR demonstrated high discrimination power to sort the ten clinical strains into six groups
according to the obtained banding pattern of DNA. The frequency of occurrence of the three
biomarkers ChuA, YjaA, and TspE4C2 among the ten uropathogenicE.coli clinical strains was 100%,
90, and 90%, respectively. The phylogenetic grouping of the ten uropathogenicE.coli strains based on
the presence of these biomarkers revealed 9 strains belonged to group B2 (subgroup B23 : chuA+,
yjaA+, TspE4.C2+) and only one strain belonged to group D (subgroup D1: chuA+, yjaA-, TspE4.C2-
). The present data would support the superior potential of ERIC1b-PCR for rigorous differentiation
among uropathogenE.coli clinical strains in comparison to the potential of Clermont et al method .