Genetic Comparative Study Of Staphyolococcus Aureus Isolated From Infections And Rural Water

Abstract

One hundred specimens from wounds, burns, and Rural water were collected , wounds and burns isolates were
collected from patients laying in hospital from different age and gender . It was found that 50 isolates belong to
Staphylococcus spp., 38 isolates were identified as S. aureus from infections isolates and 12 isolates were
identified as S. aureus from rural water according to microscopic, cultural and biochemical testing. The study of
seven extracellular enzyme as virulence factors including the enzymes: urease, lipase, DNase, haemolysin,
coagulase, β-lactamase, and lecithinase. Revealed that 100% of S.aureus which isolated from infections had the
ability to produce these enzymes, while the isolates of rural water were unable to produce the enzymes DNase,
lipase, coagulase , but they were capable to produce haemolysin, urease, lecithinase, and β-lactamase the range
for production of these factors were 50 %,84,%, 7,%, and 43% respectively. 14 Staphyolococcus isolates from
infections isolates and 4 isolates from rural water isolates were selected according of their ability for production
most of studies virulence enzymes for detection of genes encoding for the enzymes heamolysine (hly) and (coa)
by using of polymerase chain reaction (PCR) technique. The Gene hIy was detected in all isolates from
infections and rural water and coa was detected in infections isolates only.