The CD64 Expression on Neutrophils as a Diagnostic Biomarker of Bacterial Infection in Critically III Children

Abstract

Background: Bacterial infections are an important cause of hospital morbidity and mortality
worldwide. The quantification of CD64 expression on the surface of neutrophils by flow cytometry has
recently been proposed as a diagnostic test for sepsis. The aim of this study to assess the role of CD64
expression on neutrophils as diagnostic marker of bacterial infection. Patients and Methods: A crosssection study included 70 cases with evidence of sepsis in age of 2month to 13 years and conducted in
pediatric intensive care unit in Zagazig Universty Hospital. All patients in this study had been
subjected to complete history taking and clinical examination. Laboratory investigation. Results: The
present study showed mean temperature of the studied patients was 37.717 °c and mean PsO2 was
97.27 %. Pneumonia occurred in 81.4% of the studied patients and UTI occurred 10% of the studied
patients. CNS infection occurred in 5.7%, and gastroenteritis occurred in 2.9% of the studied patients.
Mean hemoglobin level among the studied patients was 9.723 g/dL. Mean RBCs and hematocrit were
3.811 (106
/mm3
) and 28.704 respectively. Mean TLC was 16.657 103
/mm3
. Platelet count ranged from
21 to 777 103
/mm3 with median182.5 103
/mm3
. Concerning differential leucocytic count, median
neutrophil, lymphocyte, monocyte, eosinophil, and basophil were 8, 3.3, 1.2, 0.15 and 0 respectively.
Positive CRP occurred in100% and Median CRP among the studied patients was 29 mg/L. Positive
PCT occurred in 88.6% and median was 0.6. Neutrophil CD 64 ranged from 69.4 to 97.13 with mean
85.916. There is statistically non-significant relation between diagnosis and neutrophil CD64 levels.
Conclusion: Use of CD64 can be included for early and more accurate diagnosis of pediatric bacterial
infection at PICU.