Impact of Butylated Hydroxyanisole on serum anti-oxidant status and histological analysis in Zebrafish Danio rerio

Abstract

The synthetic anti-oxidant Butylated Hydroxyanisole (BHA) was tested for its chronic toxicity against 
zebrafishDanio rerio. Toxicity assays initiated with finding the LD50 value of BHA against zebrafish. It was 
calculated as 54.119±3.015µM, hence the different concentration of BHA set within the LD50 value. The 
concentrations 1µM, 5µM, 10µM and 25µM of BHA were made to expose to zebrafish for 21 days continuously. 
The chronic toxicityof BHA was evaluated by the biochemical level in the serum, differential count of 
leukocytes, count of erythrocytes in the blood and histological analysis of organs live, muscle and gills. The 
toxicity of BHA revealed by biochemical alterations in the serum, abnormal count of leucocytes and 
disorganization of tissues in organs at higher concentration of BHA to fishes. The present study clearly 
demonstrates the chronic toxicity of BHA with zebrafish model.