Isolation And Characterization Of Some Bacteriophages Infecting Multi Drug Resistant Bacteria Isolated From Diabetic Foot Ulcers

Abstract

Foot ulcers are the main cause of hospitalization and mortality of diabetic patients over the world. Approximately
25 % of diabetic patients will suffer from wound infections during their lifetime. In the current study, 32 infected
diabetic foot ulcer patients in sharkia Governorate (ElAhrar hospital in Zagazig, Sharkia, Egypt and private
diabetic foot center in Fakous, Sharkia, Egypt) 64 bacterial isolates were collected during the period of October
2018 to January 2019.Resistance to antibiotics reached to 75% of these isolates .isolate no 8,25,28,29 and 30 gave
the highest resistant to antibiotics. These isolates identified as (Klebsiella pneumonia, proteus mirabilis,
Providencia rettergi, Shigella sonnei, and E.coli) .three bacterial viruses active against these bacteria were isolated
.these phages named as ØSS29, ØKP8, and ØEC30.
As ØEC30 virus active against E.coli, active against ØKP8 Klebsiella pneumonia and ØSS29 active against
Shigella sonnei.. The present study opens a new window for the application of bacteriophages as promising
antimicrobial agents against MDR bacteria.