IDEATIFICATIONFOR THE LEVEL ANTIBODES (IGG ANDIGM) OF TOXOPLASMA GONDIIIMMNOCOMPROMISEDPATIENTS IN KIRKUK CITY

Abstract

The current study is aimed to investigate the prevalence of toxoplasmosis in immunosuppressed people by
using serological methods (ELISA) and genetic methods polymerase chain reaction (PCR) it also studies their
effects on enzymes and liver functions. The study included the collection of 250 blood samples from disease
groups (patients with rheumatism who are taking immunosuppressive therapy, patients with Patients with
cancerous disease, patients with pulmonary tuberculosis, patients with diabetes and patients with
thalassemia). The study also collected samples from healthy people. We used two kits from the ELISA test,
the first for the detection of IgM antibodies and the second for the detection of IgG antibodies. The
polymerase chain reaction test was also conducted to detect the parasite using two types of primers.
Regarding biochemical tests, the level of the total protein, albumin, globulin, cholesterol, triglycerides and
glucose was detected. The results of the current study showed that the ( percentage of positive cases for
the ELISA test used to detect IgM antibodies for patients with rheumatism who are taking
immunosuppressive therapy, patients with cancerous tumors, patients with pulmonary tuberculosis,
diabetes, and patients with thalassemia were 22%, 18%, 16.6%, 20%, respectively .While the proportion of
healthy people was 3.3% and the percentage of positive cases of the ELISA test used to detect IgG
antibodies for patients with rheumatism who take immunosuppressive therapy, patients with cancerous
tumors, patients with tuberculosis, diabetes, and patients with thalassemia were 26%, 22%, 20%, 30%,
26.6%, respectively.While the proportion in healthy subjects was 10% and positive cases for both types of
antibodies were recorded for the groups, patients with cancerous tumors, patients with pulmonary
tuberculosis, diabetes patients, and patients with thalassemia were 10%, 16%, 7.5%, 13.3%, 13.3%,
respectively