Evaluating the levels of Salivary biomarker C-reactive protein in children with sleep problems and Class II malocclusion before and after twin-block therapy

Abstract

Background
Class II malocclusion is a common dental condition in children, often associated with sleep 
disturbances. C-reactive protein (CRP) is a salivary biomarker that indicates inflammation and 
may be linked to sleep problems. This study aims to assess the levels of salivary CRP in 
children with sleep problems and Class II malocclusion before and after treatment with twinblock therapy.
Materials and Methods
This prospective study included 50 children aged 8-14 years diagnosed with Class II 
malocclusion and sleep disturbances. Salivary samples were collected from each participant at 
baseline and after six months of twin-block therapy. Salivary CRP levels were measured using 
enzyme-linked immunosorbent assay (ELISA).Background
Class II malocclusion is a common dental condition in children, often associated with sleep 
disturbances. C-reactive protein (CRP) is a salivary biomarker that indicates inflammation and 
may be linked to sleep problems. This study aims to assess the levels of salivary CRP in 
children with sleep problems and Class II malocclusion before and after treatment with twinblock therapy.
Materials and Methods
This prospective study included 50 children aged 8-14 years diagnosed with Class II 
malocclusion and sleep disturbances. Salivary samples were collected from each participant at 
baseline and after six months of twin-block therapy. Salivary CRP levels were measured using 
enzyme-linked immunosorbent assay (ELISA).