BACTERIOLOGICAL PROFILE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF BLOOD CULTURE ISOLATES FROM ADULT SEPSIS PATIENTS AT A TERTIARY CARE HOSPITAL

Abstract

Background: Bacteria causing sepsis show multi drug resistance which increases morbidity and mortality in sepsis patient. The present study was conducted to identify the organisms causing sepsis in adult patients and their antibiogram. Material and methods: A total
number of 390 blood samples of adult patients with sepsis were processed by using standard microbiological procedures. Identification of organisms was carried out by using standard biochemical test and antibiotic susceptibility testing of all isolates was performed by Kirby Bauer's disc diffusion method on Mueller Hinton agar. Antibiotic sensitivity results were interpreted as per CLSI guideline. Results: Total 390 blood samples were processed in which 87(22.30%) were culture positive isolates. Out of 87 culture positive isolates, 23(26.43%) were Gram positive isolates and 60(68.96%) were Gram negative isolates. Escherichia coli and Staphylococcus aureus were predominant bacterial isolates among gram negative and gram positive bacteria respectively. Staphylococcus aureus showed maximum susceptibility to Linezolid 90% and Vancomycin 89%. E. coli isolates were susceptible to Amikacin (89%) and Cefoperazone (62.96%).