Isolation and identification of aerobic bacteria that causes otitis media and detection of their production of β-lactamases

Abstract

 The dissemination of β- lactamases production by bacteria is worldwide problem,
this study aimed to detection of β- lactamase and multidrug resistant patterns in aerobic
bacteria that causes otitis media, ear swabs collected from 53 patients attending AlHaboubi hospital in Al-Nasiriyah city during year 2019.The study found 46 out of53
ear swabs gave positive culture that distributed to 29 (90.6%) female and 18 (8.7%
male. The age group ˃50 year was the higher (12 (25.5 %)) infected with otitis media,
it appeared that patients with a chronic otitis media (COM) were more (32 (68.1%) that
than in acute otitis media (AOM) that registered 15 (31.9%). AOM appeared with a
higher rate (100%) in age group (1-10) year, while the largest age group affect by COM
was the age group ˃50 year. Fifty-five bacterial isolates distributed to 39 (70.9%)
isolated from COM and 16 (29.1%0 from AOM, Pseudomonas aeruginosa was
predominant bacteria (18(32.7%)) among other aerobic bacteria. The most antibiotic
which resistant by isolates was erythromycin, with a total resistance 89.1%, while
amikacin was the most effective with 98.2% of sensitivity. The total number of isolates
that produced β-lactamases enzymes reached 49 (89%), and Pseudomonas aeruginosa
ranked first among these isolates, as 17(34.6%) isolates were recorded, 43 (78.2%) of
isolates had multidrug resistance, the most frequency bacteria with MDR were Proteus.
spp, E. coli, Enterobacter spp. and Klebsiella spp, it registered 100%.