TO STUDY THE ACTIVITY AND THE CORRELATION OF SERUM GGT, SERUM MDA AND BLOOD GSH LEVELS IN THE PATIENTS WITH ALCOHOLIC LIVER DISEASE

Abstract

BACKGROUND: Alcoholic Liver disease is mainly of three types: - Alcoholic fatty liver; Alcoholic hepatitis; and alcoholic cirrhosis. During the metabolism of alcohol in the liver, it produces highly reactive molecules that can destroy vital cell components through a chemical
process called oxidation. Oxidative stress increases the rate of production of free radical hence induces lipid peroxidation. Antioxidants are natural defence molecules existing in our system and these molecules are capable of scavenging the deleterious free radicals.
AIMS: To study the activity of serum GGT levels, Serum MDA levels and GSH levels in whole blood in the patients with alcoholic liver disease and its comparison with controls and to study the correlation of serum MDA levels with serum GGT levels and blood GSH levels in the patients with alcoholic liver disease.
MATERIAL AND METHOD: The study was conducted on 50 diagnosed cases of alcoholic liver disease visiting Rajindra Hospital, Patiala and 50 healthy subjects of matched age & sex served as controls and Biochemical Investigations of Serum GGT levels, Serum MDA levels and GSH levels in the whole blood were conducted in the department of Biochemistry Govt. Medical College Patiala and the results were statistically analysed.
RESULTS: The mean values of serum GGT in the study group and in the control group were 20.80±6.38 (IU/L) and 234.44±204.31(IU/L) respectively (Normal value of GGT 0-50 (IU/L), mean values of serum MDA in the study group and control group were 22.00±5.03
and 53.90±11.43(µmol/L) respectively and mean values of blood glutathione in the control group and the study group were 41.6±4.51 and 18.40±2.39(mg%) respectively. The decrease mean blood Glutathione levels in the study group as compared to mean blood Glutathione levels in the control group shows statistically significant association (p value < 0.001), the mean value of serum MDA Levels in the study group was elevated as compared to control group and shows statistically significant association (P value <0.001) and the mean serum GGT Levels in the study group was significantly elevated as compared to the control group (234.45±204.31IU/L Vs 20.80+6.38 IU/L; P<0.001)
CONCLUSION: Our study shows that there was increased levels of Serum MDA, Serum GGT and decreased levels of blood glutathione in the study group and there was a positive correlation between Serum MDA levels and Serum GGT levels in the study group and there
was a negative correlation between serum MDA levels and blood glutathione levels in the study group. This correlation predicts that during increased oxidative stress, the GSH was utilized to counter the oxidative stress due to alcoholic liver disease.