Comparative analysis of the effects of temperature variations on the tissue-dissolving capacities of chlorine dioxide, calcium hypochlorite, and sodium hypochlorite

Abstract

Aim: Using isotonic saline as a benchmark, this study evaluated the tissue dissolving ability of 3% sodium hypochlorite (NaOCl) alongside two other irrigants—10% calcium hypochlorite and 13.8% chlorine dioxide (ClO2)— across different temperatures. Material: Methodology: For this study, weight-adjusted bovine muscle tissue specimens (50 ± 5 mg) were prepared. A total of 120 frozen tissue samples were evenly distributed among four groups. The experiments were conducted at three different temperature levels: room temperature, 37°C, and 45°C. Each group contained 30 tissue samples, which were immersed in 5 mL of the designated test solution at the specified temperature for 20 minutes, with the solution being replaced every two minutes. After the experiment, the tissues were carefully removed, dried using absorbent paper, and weighed with a precision scale. The percentage of weight loss for each specimen over the course of the experiment was then recorded.