Abstract

A UPLC-MS method for the quantification of the levalbuterol is described, in addition to its application to the in vitro study of metabolism in rat liver microsomes. Protein precipitation extraction was used to extract the sample from microsome samples and the separation was performed on a C18 protected with a guard column of the same type using Water: Acetonitrile with 0.1% Formic acid as the mobile phase, at a flow rate of 0.3mlmin−1. The detection was carried out at 276 nm. The method proved to be linear in the range of 2.5-30 ngml−1, with quantification Precision and accuracy, demonstrated by within-day and between-day assays, were lower than 15%. The metabolic study demonstrated that metabolism found two metabolites formed in the incubation mixture of liver microsomes and sample with NADPH, which are identified by LC-MS.

Description

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