Abstract

Background:Hematoxylin and Eosin (H&E) staining is a fundamental technique in histopathology, allowing for the visualization of tissue structures and pathological alterations. Standardizing H&E staining protocols for human liver sections with varying thicknesses and time intervals is crucial for accurate histopathological diagnoses. Aim and Objective: This study aimed to investigate the standardization of H&E staining protocols for human liver sections with different thicknesses and time intervals in histopathology studies. Materials and Methods: This Experimental study utilized human liver tissue samples from a total of 50 patients, with each thickness-time interval combination having 10 replicates. Liver tissue specimens were obtained from surgical resections and biopsies. Sections of varying thicknesses (5μm, 10μm, and 15μm) were prepared using a microtome. H&E staining was performed with different time intervals (30, 60, and 90 minutes). Stained sections were evaluated for staining quality, intensity, and clarity under light microscopy. Statistical analysis was conducted to assess significant differences between groups. Results: Optimal staining quality was observed in liver sections with a thickness of 10μm stained for 60 minutes. Statistical analysis confirmed significant differences in staining quality based on both thickness and staining duration. These findings contribute to refining staining protocols, ultimately improving the accuracy of pathological diagnoses in liver diseases.

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