Abstract

Aim: The aim of the present study was to identify the risk factors of anaesthesia workstation contamination in different operation theatres. Material & methods: Total 120 samples were taken from different sites and equipment’s of 5 OTs. Sterile swabs in nutrient broth were used to collect samples. They were placed back into the broth after collection. All the samples were labelled properly and immediately transported to the Microbiology laboratory and incubated 37°C for 4 hours. Swabs taken from different sites were inoculated on Blood agar and MacConkey agar. These culture plates were incubated at 37°C under aerobic condition for 24 hours. Isolation and identification of isolates were done as per standard guidelines. Results: In the category of before decontamination, 40% of the samples are reported in General surgery OT, 20% of them are reported in ENT surgery, 20% of them are reported in Ortho surgery and another 20% of them are in the Oncology surgery OT In the category of before decontamination, 25% of the samples are reported with APL, 25% of them are reported with Oxygen Flowmeter, 25% of them are reported with Vaporizer Dial and another 25% of them are reported with Workstation Desk surface. 30% of the samples are reported with positive growth and 70% of them are not reported with sample positive growth. 13.3% of the samples are reported with low level of decontamination, 6.7% of the samples are reported with intermediate level of decontamination and 80% of the samples are not reported with decontamination. It was concluded from the analysis that OT name and Decontamination are not associated. Conclusion: Anaesthesia workstation contamination is a not rare contamination. Pre wash swab collection decontamination is associated with high incidence of contamination with surgical sites infections as compared to post wash swab collection decontamination is associated with low incidence of contamination with surgical site infections.

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