Abstract

Background: Objectives: Primary Objective: To compare the ESR estimate by an automated analyser (ALI FAX Roller 20LC) and westergren method. Secondary Objective: To compare the ESR estimate by an automated analyser and westergren method in individuals with Tuberculosis, with Anaemia, in pediatric patients, and Male and female individuals with chronic inflammatory diseases. Materials and Methods: Blood is collected in black sodium citrate (3.8%) vacutainer under aseptic precautions. Then it is measured in westergren tube (mm per hour) and then same sample will be run in the Automated ESR analyser (ALI FAX Roller 20LC) in which reading is available in 30 mins. Results: The ESR measured by Westergren method ranged from 12mm/1st hour. to 127 mm/1st hour, with a mean of 46.76± 22.650 mm/1st hour. while ESR measured by automated method ranged from 7.8 mm/1st hour to 128.7 mm/1st hr. witha mean of 45.165±23.3753 mm/1st hour. There was significantly strong correlation between automated and the Westergren method for determination of ESR (r =0.933; p = <0.001). Similar results were observed across all ages, gender, anemic and Tuberculosis patients. Conclusion: Automated and the Westergren method are strongly correlated. Automated method can be used in place of Westergren method for determination of ESR.

Description