ISSN 0975-3583

Journal of Cardiovascular Disease Research

    Comparative evaluation of detection rate and isolation of Mycobacterium tuberculosis using LJ medium, MGIT 960 and Smear microscopy from pulmonary and extrapulmonary Tuberculosis

    Lautika Sonkar, Deeksha Yadav, Rampal Singh Journal of Cardiovascular Disease Research ISSN:0975-3583,0976-2833 VOL14,ISSUE05,2023
    JCDR. 2023: 857-864


    Aim: To compare the recovery rate and time for isolation of Mycobacterium tuberculosis from clinical samples by MGIT 960 and conventional Lowenstein-Jensen medium (LJ). Materials and methods: This cross-sectional study was carried out on 80 samples which were clinically suspected cases of pulmonary and extra-pulmonary tuberculosis. Samples were processed for direct Ziehl-Neelsen staining (ZN), culture in MGIT 960 and LJ respectively. Results: Out of 80 samples, 20 cases were positive by either of the methods. The positive samples for ZN staining, LJ medium and MGIT 960 were (12, 15%), (18, 22.5%) and (20, 25%) respectively. Among the tuberculosis positive cases, (51, 63.75%) were female and 29, 36.25%) were male, with peak incidence (43.47%) in age group of 21-40 years. The sensitivity, specificity and positive predictive value of MGIT 960 compared to LJ medium was 100%, 96.77% and 90% respectively. MGIT 960 showed faster rates of isolation (1-10 days) as compared to LJ medium (11-50 days). Conclusion: Liquid culture medium (MGIT 960) is more accurate and rapid for the diagnosis of TB. So, it can be used in combination with LJ medium for rapid detection and early treatment of TB



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    Volume & Issue

    Volume 14 Issue 5