Abstract

Tuberculosis (TB) is global public health problem, a leading cause of morbidity and mortality predominantly in developing countries. Many detection methods have been developed in the recent years. Mycobacteria Growth Indicator Tube (MGIT 960) is more precise and rapid for microbiological identification of Mycobacterium tuberculosis in clinical samples to prevent the spread of infection and to accelerate the effective treatment. Aim: To compare the recovery rate and time for isolation of Mycobacterium tuberculosis from clinical samples by MGIT 960 and conventional Lowenstein-Jensen medium (LJ). Materials and methods: This cross-sectional study was carried out on 80 samples which were clinically suspected cases of pulmonary and extra-pulmonary tuberculosis. Samples were processed for direct ZiehlNeelsen staining (ZN), culture in MGIT 960 and LJ respectively. Results: Out of 80 samples, 20 cases were positive by either of the methods. The positive samples for ZN staining, LJ medium and MGIT 960 were (12, 15%), (18, 22.5%) and (20, 25%) respectively. Among the tuberculosis positive cases, (51, 63.75%) were female and 29, 36.25%) were male, with peak incidence (43.47%) in age group of 21-40 years. The sensitivity, specificity and positive predictive value of MGIT 960 compared to LJ medium was 100%, 96.77% and 90% respectively. MGIT 960 showed faster rates of isolation (1-10 days) as compared to LJ medium (11-50 days). Conclusion: Liquid culture medium (MGIT 960) is more accurate and rapid for the diagnosis of TB. So, it can be used in combination with LJ medium for rapid detection and early treatment of TB

Description

.