Abstract

The effectiveness of ELISA (enzyme-linked immunosorbent assay) and immunofluorescence tests for the diagnosis of autoantibodies among antenatal mothers is to detect the presence of autoantibodies in pregnant women. Autoantibodies are antibodies that mistakenly target and attack the body's own tissues and organs, leading to autoimmune diseases. Aim and objective: compare the effectiveness of ELISA and immunofluorescence tests for the diagnosis of autoantibodies among antenatal mothers. Material and method: Both testing methods were applied to 200 samples. Of these, 100 samples were from the test group, and 100 samples were controls (negative and positive controls). Of the 100 test samples. Observation and Results:The study revealed that 70% of the cases yielded a positive result when subjected to the ELISA method, whereas 71% of the cases tested positive when subjected to the IFAT method. The ELISA method yielded a positive result in 5% of individuals in the control group, whereas the IFAT method yielded a positive result in 6% of the same group. Conclusions: The findings of this study suggest that the use of indirect immunofluorescence assays (IFA) is more effective than enzyme-linked immunosorbent assays (ELISA) for the purpose of ANA testing.

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