Abstract

Staphylococcus genus comprising Staphylococcus aureus and coagulase negative Staphylococcus (CoNS) are widely distributed in nature and can infect diversity of hosts. Staphylococcus is the major pathogen causing biofilm associated infections caused by contaminated hospital indwelling devices. Aim: 1. To detect and compare the prevalence of biofilm producer and nonproducer Staphylococcus isolated from clinical materials by two different methods, viz. tissue culture plate (TCP) method and tube method (TM) 2. Antibiotic sensitivity pattern in all these isolates is observed. Material and Methods: A total of 95 clinical Staphylococcus aureus isolates were collected from Jan 2021 to Jan 2022. Staphylococcus were isolated and identified from various clinical samples by standard microbiological techniques. The in vitro biofilm production was measured using, TM and TCP methods. Result: Specimens belonging to age range: 3 - 80 years were collected; M: F – 1: 1.8. Out of 95 isolates studied, 49 were MSSA,44 were MRSA, 2 were CONS. In a total of 95 samples, 35(37%) was found to form biofilm by tissue culture plate method while tube method detected biofilm in 22(23%). 34 % of MRSA and 14% MSSA were biofilm producers. The samples were 100% sensitive to linezolid, vancomycin and Teicoplanin. Conclusion: Both methods, TCP and TM showed that Staphylococcus isolates have high degree of biofilm-forming ability, the tissue culture method had a higher sensitivity. Surveillance of biofilm formation by S. aureus may help in management of infections in cancer patients.

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