Journal of Cardiovascular Disease Research
Early detection of dengue by ELISA
Lahiri Somsubhra, 2Narmadha E, Reddy Spoorti Channa
JCDR. 2023: 460-462
Abstract
It is essential to have an accurate and early diagnosis of dengue in order to diagnose dengue virus infections in a timely manner. Through the utilisation of NS1 antigen and IgM antibody as a fast diagnostic tool, the purpose of this work is to identify dengue in its earliest stages. In all, 108 serum samples were collected for the IgM Ab detection of dengue virus infection, whereas 101 serum samples were collected for the NS1 Ag detection of dengue virus infection. Using independent ELISA kits, these samples were analysed to determine whether or not they contained NS1 antigen and IgM antibodies. 19 out of 101 samples were positive for the presence of NS1.There were 33 samples that tested positive for IgM out of a total of 108. There were 36 positive results across both the 101 and 108 samples. According to the findings, the ELISA method that was used is suitable for the early detection of dengue and has the potential to boost the diagnostic effectiveness for early diagnosis.
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