Abstract

The study's objective is to provide a rapid, simple, accurate, and cost-effective RP-HPLC assay technique for the simultaneous quantification of domperidone and cinnarizine in pharmaceutical bulk. Domperidone and cinnarizine in bulk have been successfully separated using this approach. Separation was carried out at 226 nm on a C18 SunfireTM (5 m, 250 mm 4.6 mm) analytical column using an Orthophosphoric acid and acetonitrile (60:40) mobile phase operating in isocratic elution mode at a flow rate of 1.0 ml/min. Domperidone had a retention time of 2.52 minutes, whereas Cinnarizine's was 5.18 minutes. PDA detection at 226 nm based on peak area with linear calibration curve in concentration ranges of 0-5 g/ml (0.9999) and 0-25 g/ml (0.9996) allowed for accurate quantitation of Domperidone and Cinnarizine, respectively. Domperidone and cinnarizine had LODs of 0.3325 and 0.615 g/ml, respectively. The suggested approach is well-suited for use in quality-control labs for bulk quantitative analysis of the medications, both alone and in combination, since it is straightforward and quick while maintaining high accuracy and precision.

Description