Abstract

Introduction: Acinetobacter species is one of the most frequent opportunistic pathogen responsible for serious infection in intensive care unit. Acinetobacter species most often multidrug resistant is a difficult to treat pathogen particularly in ICU. The aim of our study to identified phenotypic characterization of multidrug resistance Acinetobacter species with special reference to metellobetalactamase production from various clinical isolates in tertiary care hospital. Methods: The Present study was conducted in the department of Microbiology on 210 isolates of Acinetobacter species recovered from various clinical samples. This study was approved by institutional ethical committee. The isolates were identified as per standard conventional method. Antimicrobial susceptibility test were processed as per standard CLSI guideline. The resistance isolates were screened and confirmed by modified hodge test and double disk synergy test. Results: Out of 210 Acinetobacter species isolated from various clinical sample 92.8% was A.baumanii followed by A. lwoffi (5.8%), A Haemolyticus (1.4%). 84% species were carbapenem resistance. Out of 84% carbapenem resistance strain 58 were positive by modified hodge test and 49 were MBL positive by IMP-IMP double disk synergy test. Conclusion: In this study can be concluded that emergence of Acinetobacter spp. alarming threat and excessive use of carbapenem drug therefore early detection and prompt infection control measures is important to prevent spread of MBL to other gram negative bacteria.

Description

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