Abstract

Phytochemicals and natural products are plant derived secondary metabolites, which may exert many biological activities in humans, including anticancer properties. Certain natural and synthetic compounds that can modulate immune responses in a positive or negative manner are known as Immunomodulators. The best types of nanoparticles used for drug delivery and the biodistribution of phytochemicals and natural products include polymer nanoparticles, solid lipid nanoparticles (SLNs), crystal nanoparticles, liposomes, micelles, and dendrimers We tried various solvent for extractions i.e. Petroleum ether (40-60°c), chloroform, ethanol, water but we get better yield in ethanol. The extracts obtained were then subjected to qualitative chemical examination for the identification of various plant constituents. The various tests and reagents used i.e. Test for Carbohydrates, Test for Proteins, Test for Fats and Oils, Test for Steroids, Test for Glycosides, Test for Alkaloids, Test for Flavonoids, Tests for Tannins and Phenolic Compounds. The ethanol extract of Annona Squmosa (ASA), Momordica Dioica (MDA) and Withania Somnifera (WSA) showed the presence of major phytoconstituents and used as successive ethanol extract (SEE) for in-vivo studies. The results of the acute toxicity study indicated that the LD50 of the extracts was more than 2000 mg/Kg body weight. The in-vivo study results indicate that the extract has a greater effect on the early hypersensitivity reaction and a less pronounced effect on the delayed hypersensitivity reaction.

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