Abstract

Nucleolar organizing regions (NOR) can be located by staining with silver nitrate under prescribed conditions; the structures thus demonstrated termed as `AgNORs'. Nucleolar organizing regions (NOR) counts might be of helpful specifically in conditions where histological distinction of prostatic cancer is difficult. Present study was aimed to study role of AgNORs in differentiating benign from malignant lesions of prostate. Material and Methods:Present study was single-center, prospective, observational study, conducted in samples of prostatic biopsies. The diagnosis in all cases were made on H & E and subsequently taken up for study.All prostatic biopsies were stained by the modified AgNOR technique. All silver-stained structures, both intra and extra nucleolar were counted.Results: Based on the histopathological examination of prostatic biopsies, 87 were diagnosed as benign (BPH) and 13 cases were malignant (Carcinoma prostate). Maximum no. of cases of BPH are in the age group of 51 - 60 years (40.2 %) followed by 61-70 years (39.1 %). Majority cases of carcinoma prostate were from age group of 51 - 60 years (38.46 %). In the present study we have applied AgNOR staining procedure to histopathologically diagnosed cases of BPH and Ca prostate. In BPH showed a mean AgNOR count of 2.38 ± 0.31, while biopsies from carcinoma prostate had a mean AgNOR count of 5.39 ±0.408. In our study BPH constituted majority of the lesions (87% of cases). Among BPH lesions, BPH with prostatitis constituted (29 %) which obscures the AgNOR staining. Ca prostate constituted (13%). Conclusion: Prostatic disease is responsible for significant morbidity and mortality in men throughout the world. AgNOR is a simple, cost effective and easy stain to evaluate the proliferative activity of different prostatic lesions

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