Journal of Cardiovascular Disease Research
Study of haemoglobinopathies in paediatric age group by high performance liquid chromatography in a tertiary care hospital in Sonitpur district of Assam
Dr. Geetanjali Gogoi, Dr. Amrit Sarmah, Dr. Partha Pratim Talukdar, Dr. Barnali Das, Dr. Lekhraaj Gautam
JCDR. 2023: 2062-2066
Abstract
Inherited haemoglobin disorders which includes disease like thalassemia and other haemoglobinopathies are a major health burden accounting not only for significant morbidity and mortality but also implies a social and financial burden. Aims and Objectives: This prospective cross-sectional study was undertaken with an aim to determine the prevalence and distribution of different haemoglobinopathies in paediatric age group. Method: A total of 500 suspected cases of haemoglobinopathies refereed to haematology section of Pathology Department were included in the study. CBC and PBS examination was done for each case followed by haemoglobin typing, which was done by High Performance liquid Chromatography (BIORAD D10) based on the principle of cation exchange. Results: The prevalence of Hemoglobinopathies was found to be 49.8% in the present study with Hb E Trait being the most common abnormal haemoglobin detected followed by Hb E Disease and Hb Eβthalassemia compound heterozygous. Conclusion: Cation Exchange High Performance Liquid Chromatography (CE-HPLC) is very effective, reliable and highly reproducible method for detection of abnormal haemoglobin
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