Abstract

The aim of present study is to evaluate the prevalence of anticardiolipin antibodies among women with recurrent abortions and to determine any relation between anticardiolipin antibodies and number of abortions and their gestational age of abortions. An indirect Non-competitive enzyme immunoassay for the semi quantitative and qualitative determination of cardiolipin IgG antibodies is performed. The wells of the microtitre plate are coated with cardiolipin antigen. Antibodies specific for cardiolipin present in the patient’s sample bind to the antigen. The second step, the antigen antibody complex reacts with an enzyme labelled second antibody (Enzyme Conjugate) which leads to the formation of an enzyme labelled antigen antibody sandwich complex. The enzyme label converts added substrate from a colored solution

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