Abstract

Staphylococcus aureus is a gram-positive spherical bacterium commonly present in the nasal passages, respiratory system, and skin. The aim and objectives of this investigation were to identify phenotypic and genotypic resistance in Staphylococcus aureus and to isolate it. Materials and Methods: This study was cross-sectional and prospective. Isolates were collected from different clinical samples for this study conducted at the Department of Microbiology, Madha Medical College and Research Institute in Chennai, Tamil Nadu, India. The study was conducted from September 2017 to August 2018. Results: Staphylococcus aureus is a significant pathogen that colonises around 30% of asymptomatic individuals. Drug resistance has developed as a result of the growing unintentional utilisation of potent antibiotics, leading to the rapid emergence of multidrug-resistant microorganisms. Real-time PCR offers great sensitivity and a significantly shorter turnaround time compared to previous procedures. Laboratories equipped with molecular testing facilities for Real-time PCR can utilise it as a standard diagnostic technique. Conclusion: Improper antibiotic use has led to extensive resistance. Novel chemicals have been introduced, leading to the inadvertent development of resistance pathogens by doctors.

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